Multiple Sulfur Acceptors Dock at IscS

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Multiple Sulfur Acceptors Dock at IscS

Like a busy dock, the enzyme IscS offers a temporary berth to multiple protein vessels where they can load up with their cargo of sulfur atoms. The sulfur is then incorporated into essential biological structures such as the iron–sulfur clusters that catalyze oxidation–reduction reactions in the electron transport chain. Although the structure of IscS has been known for some time, it was not cl...

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The cysteine desulfurase IscS is a highly conserved master enzyme initiating sulfur transfer via persulfide to a range of acceptor proteins involved in Fe-S cluster assembly, tRNA modifications, and sulfur-containing cofactor biosynthesis. Several IscS-interacting partners including IscU, a scaffold for Fe-S cluster assembly; TusA, the first member of a sulfur relay leading to sulfur incorporat...

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Transfer of sulfur from IscS to IscU during Fe/S cluster assembly.

The cysteine desulfurase enzymes NifS and IscS provide sulfur for the biosynthesis of Fe/S proteins. NifU and IscU have been proposed to serve as template or scaffold proteins in the initial Fe/S cluster assembly events, but the mechanism of sulfur transfer from NifS or IscS to NifU or IscU has not been elucidated. We have employed [(35)S]cysteine radiotracer studies to monitor sulfur transfer ...

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ژورنال

عنوان ژورنال: PLoS Biology

سال: 2010

ISSN: 1545-7885

DOI: 10.1371/journal.pbio.1000353